Ziege anti-Human IgA (Heavy Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN100717

Produktdetails

Target: IgA

Bindungsspezifität: Heavy Chain

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Human

Produktmerkmale: Anti-Human IgA alpha heavy chain antibody generated in goat detects specifically Human IgA alpha heavy chain.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Human IgA alpha heavy chain antibody was produced by repeated immunization with Human IgA alpha heavy chain in goat.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-HUMAN IgA (alpha chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:60,000 - 1:600,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen

Yu, Pollock, Duval, Lewis, Joseph, Meade, Cavacini: "Neutralization of HIV by milk expressed antibody." in: Journal of acquired immune deficiency syndromes (1999), Vol. 62, Issue 1, pp. 10-6, (2013) (PubMed).

Antigendetails

Target: IgA

Abstract: IgA Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-Human IgA (alpha chain) biotin conjugated Antibody, goat anti-Human IgA alpha Antibody biotin conjugation

Background: Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.