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Ziege anti-Human IgA, IgG, IgM Antikörper (Alkaline Phosphatase (AP))

Rockland 609-105-130 ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
Produktnummer ABIN100789
Hersteller Produkt- Nr.: 609-105-130
  • Target Alle IgA, IgG, IgM Produkte
    IgA, IgG, IgM
    Reaktivität
    • 71
    • 49
    • 34
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Wirt
    • 160
    • 55
    • 4
    • 3
    • 2
    Ziege
    Klonalität
    • 216
    • 2
    Polyklonal
    Konjugat
    • 54
    • 38
    • 33
    • 27
    • 22
    • 10
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Alkaline Phosphatase (AP)
    Applikation
    • 116
    • 112
    • 78
    • 77
    • 69
    • 56
    • 26
    • 23
    • 22
    • 22
    • 18
    • 17
    • 16
    • 15
    • 12
    • 9
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Hersteller Produkt- Nr.
    609-105-130
    Hersteller
    Rockland
    Verwendungszweck
    Human IgG IgA IgM (H&L) Antibody Alkaline Phosphatase Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.  This reagent is suitable for the detection of all human immunoglobulin isotypes, subclasses and chain combinations.
    Produktmerkmale
    Anti-Human IgG (H&L) Alkaline Phosphatase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
    Aufreinigung
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterilität
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Human IgG, IgA and IgM whole molecule
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Human IgG IgA IgM Alkaline Phosphatase conjugate has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.1 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Haltbarkeit
    12 months
  • Target
    IgA, IgG, IgM
    Andere Bezeichnung
    IgG,IgA,IgM (IgA, IgG, IgM Produkte)
    Substanzklasse
    Antibody
    Hintergrund
    Anti-Human IgG IgA IgM (H&L) Alkaline Phosphatase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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