Ziege anti-Human IgA, IgG, IgM Antikörper (FITC)

Produktnummer ABIN100791

Hersteller Produkt- Nr.: 609-102-130

Produktdetails

Target: IgA, IgG, IgM

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Hersteller Produkt- Nr.: 609-102-130

Hersteller: Rockland

Verwendungszweck: Human IgG IgA IgM (H&L) Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human isotypes and chain combinations.

Produktmerkmale: Anti-Human IgG (H&L) Fluorescein generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Aufreinigung: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.

Immunogen: Optional[Immunogen]: Human IgG, IgA and IgM whole molecules

Isotyp: IgG

Labeling Ratio: 2.91

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG,IgA,IgM (IgA, IgG, IgM Produkte)

Substanzklasse: Antibody

Hintergrund: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.