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Ziege anti-Esel IgG (Heavy & Light Chain) Antikörper (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
Produktnummer ABIN101127
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2381
    • 511
    • 424
    • 207
    • 131
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 723
    • 702
    • 604
    • 483
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 84
    • 58
    • 48
    • 35
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Esel
    Wirt
    • 1790
    • 1576
    • 574
    • 244
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Ziege
    Klonalität
    • 3449
    • 178
    • 1
    Polyklonal
    Konjugat
    • 589
    • 417
    • 417
    • 383
    • 282
    • 276
    • 244
    • 119
    • 108
    • 67
    • 66
    • 64
    • 61
    • 58
    • 52
    • 51
    • 48
    • 46
    • 45
    • 44
    • 41
    • 39
    • 36
    • 33
    • 33
    • 31
    • 30
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    FITC
    Applikation
    • 1956
    • 1851
    • 1810
    • 1522
    • 933
    • 909
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 54
    • 53
    • 31
    • 28
    • 25
    • 20
    • 18
    • 15
    • 11
    • 9
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Spezifität
    IgG (H&L)
    Kreuzreaktivität
    Esel
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Donkey IgG whole molecule

    Isotyp
    IgG
    Labeling Ratio
    2.3
  • Applikationshinweise

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Kommentare

    Excitation/Emission wavelength: 494 nm/514 nm

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Product is photosensitive and should be protected from light.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: goat anti-Donkey IgG Antibody fluorescein conjugation, goat anti-Donkey IgG FITC conjugated Antibody

    Background: Anti-Donkey IgG Fluorescein Antibody generated in goat detects donkey IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

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