Kaninchen anti-Ziege IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN101250

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG F(c)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: immunoaffinity chromatography using Goat IgG coupled to agarose beads

Sterilität: Sterile filtered

Immunogen:

Immunogen: Goat IgG F(c) fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Goat IgG F(c) Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:4,000

Western Blot Dilution: 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Rabbit Anti-Goat IgG F(c) Antibody Alkaline Phosphatase Conjugation, Rabbit Anti-Goat IgG Fc Fragment Alk Phos conjugated Antibody

Background: Anti-Goat IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of goat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.