Ziege anti-Armenischer Hamster IgG (Heavy & Light Chain) Antikörper

Produktnummer ABIN101376

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Armenischer Hamster

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Spezifität: IgG (H&L)

Kreuzreaktivität: Armenischer Hamster

Produktmerkmale: Concentration Definition: by Refractometry

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Armenian Hamster IgG and Armenian Hamster Serum.  Minimal cross reactivity is expected against golden syrian hamster IgG.

Immunogen:

Immunogen: Armenian Hamster IgG whole molecule

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Secondary antibody to hamster IgG is available in a variety of formats. Anti-IgG Secondary Antibody is suitable for western blot, ELISA, ChIP and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

ChIP Dilution: User Optimized

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Secondary Antibody, goat Anti-Hamster IgG, goat Anti-Armenian Hamster IgG

Background: Anti-Armenian Hamster IgG Antibody generated in goat detects Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.