Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (Biotin)
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Biotin
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Hersteller Produkt- Nr.
- 609-1602
- Hersteller
- Rockland
- Verwendungszweck
- Human IgG (H&L) Antibody Biotin Conjugated
- Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Human IgG, and Human Serum.
- Produktmerkmale
- Anti-Human IgG (H&L) Alkaline Phosphatase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
- Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
- Optional[Immunogen]: Human IgG whole molecule
- Isotyp
- IgG
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- Applikationshinweise
- Application Note: Anti-Human IgG (H&L) Biotin Conjugate has been tested by ELISA and is assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Konzentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions." in: The European journal of neuroscience, Vol. 33, Issue 8, pp. 1447-61, (2011) (PubMed).
: "
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Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions." in: The European journal of neuroscience, Vol. 33, Issue 8, pp. 1447-61, (2011) (PubMed).
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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