Kaninchen anti-Human IgG (F(ab')2 Region) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN101631

Hersteller Produkt- Nr.: 609-4318

Produktdetails

Target: IgG

Bindungsspezifität: F(ab')2 Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 609-4318

Hersteller: Rockland

Verwendungszweck: Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum.  No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

Produktmerkmale: Anti-Human IgG (H&L) Peroxidase generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Aufreinigung: Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Human IgG F(ab')2 fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human IgG F(ab')2 Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 μg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product. This product is also suitable for Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Anti-Human IgG F(ab')2 Peroxidase Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.