Kaninchen anti-Human IgG (Fc Region) Antikörper (FITC)
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Fc Region
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- IgG F(c)
- Produktmerkmale
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Concentration Definition: by UV absorbance at 280 nm
Fluorochrome/Protein Ratio: 3.2 moles DTAF per mole of Rabbit IgG
Excitation Wavelength: 495 nm
Label Molecular Weight: 390
Emission Wavelength: 528 nm - Aufreinigung
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
- Immunogen
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Immunogen: Human IgG F(c) fragment
- Isotyp
- IgG
- Labeling Ratio
- 3.2
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- Applikationshinweise
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Application Note: Anti-Human IgG F(c) Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Product is photosensitive and should be protected from light.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: rabbit anti-Human IgG F(c) Fluorescein Conjugated Antibody, rabbit anti-Human IgG F(c) fragment FITC Conjugated Antibody, rabbit anti-Human IgG Fc Antibody Fluorescein Conjugation, rabbit anti-Human IgG Fc fragment Antibody FITC Conjugation
Background: Anti-Human IgG F(c) Fluorescein Conjugated generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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