Ziege anti-Affe IgG Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN101677

Hersteller Produkt- Nr.: 617-105-012

Produktdetails

Target: IgG

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 617-105-012

Hersteller: Rockland

Verwendungszweck: Monkey IgG (gamma chain) Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Monkey IgG and Monkey Serum. 

Produktmerkmale: Anti-Monkey IgM (mu chain) alkaline phosphatase antibody generated in goat specifically detects monkey IgM.

Aufreinigung: Monkey IgG gamma chain specific antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Monkey IgG gamma heavy chain

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Monkey IgG alkaline phosphatase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Referenzen

Pollara, Jones, Huffman, Edwards, Dennis, Li, Jha, Goodman, Kumar, LaBranche, Montefiori, Fouda, Hope, Tomaras, Staats, Ferrari, Permar: "Bridging Vaccine-Induced HIV-1 Neutralizing and Effector Antibody Responses in Rabbit and Rhesus Macaque Animal Models." in: Journal of virology, Vol. 93, Issue 10, (2019) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Anti-Monkey IgG alkaline phosphatase conjugated antibody generated in goat detects specifically Monkey IgG. This secondary alkaline phosphatase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.