Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN101744

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Spezifität: IgG (H&L)

Produktmerkmale: Anti-Mouse IgG whole molecule antibody generated in goat detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Gentamicin sulfate

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C

Referenzen

Hangas, Kekäläinen, Potter, Michell, Aho, Rutanen, Spelbrink, Pohjoismäki, Goffart: "Top3α is the replicative topoisomerase in mitochondrial DNA replication." in: Nucleic acids research, Vol. 50, Issue 15, pp. 8733-8748, (2022) (PubMed).

Hangas, Aasumets, Kekäläinen, Paloheinä, Pohjoismäki, Gerhold, Goffart: "Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2." in: Nucleic acids research, Vol. 46, Issue 18, pp. 9625-9636, (2019) (PubMed).

Gerber, Squires, Hepler: "14-3-3γ binds regulator of G protein signaling 14 (RGS14) at distinct sites to inhibit the RGS14:Gαi-AlF4- signaling complex and RGS14 nuclear localization." in: The Journal of biological chemistry, Vol. 293, Issue 38, pp. 14616-14631, (2019) (PubMed).

Gallardo, Arias-Darraz, Cárcamo: "Effect of Breed on Transcriptional and Protein Expression of Lipogenic Enzymes in Tail and Subcutaneous Adipose Tissue from Two Grazing Breeds of Lambs." in: Animals : an open access journal from MDPI, Vol. 9, Issue 2, (2019) (PubMed).

Herbers, Kekäläinen, Hangas, Pohjoismäki, Goffart: "Tissue specific differences in mitochondrial DNA maintenance and expression." in: Mitochondrion, Vol. 44, pp. 85-92, (2019) (PubMed).

Lin, Dong, Wang, He, Wei, Wu, Wang: "Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)." in: Molecular medicine reports, Vol. 20, Issue 2, pp. 1819-1825, (2019) (PubMed).

Sanderson, Mikhael, Ramesh, Dai, Locasale: "Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cells." in: Science advances, Vol. 5, Issue 6, pp. eaav7769, (2019) (PubMed).

Spasojevic, Marangoni, Vacher, Assayag, Meseure, Château-Joubert, Humbert, Karam, Ricort, Auclair, Regairaz, Bièche: "PKD1 is a potential biomarker and therapeutic target in triple-negative breast cancer." in: Oncotarget, Vol. 9, Issue 33, pp. 23208-23219, (2018) (PubMed).

Ray, Wilkinson, Paul: "Regulation of Trypanosoma brucei Acetyl Coenzyme A Carboxylase by Environmental Lipids." in: mSphere, Vol. 3, Issue 4, (2018) (PubMed).

Elsaeidi, Macpherson, Mills, Jui, Flannery, Goldman: "Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 38, Issue 9, pp. 2246-2261, (2018) (PubMed).

Liberti, Dai, Wardell, Baccile, Liu, Gao, Baldi, Mehrmohamadi, Johnson, Madhukar, Shestov, Chio, Elemento, Rathmell, Schroeder, McDonnell, Locasale: "A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product." in: Cell metabolism, Vol. 26, Issue 4, pp. 648-659.e8, (2018) (PubMed).

Neiser, Koskenkorva, Schwarz, Wilhelm, Burckhardt: "Assessment of Dextran Antigenicity of Intravenous Iron Preparations with Enzyme-Linked Immunosorbent Assay (ELISA)." in: International journal of molecular sciences, Vol. 17, Issue 7, (2017) (PubMed).

Mendez, Alfaro, Morales-Soto, Dar, McCullagh, Gotthardt, Li, Acosta-Alvear, Sidrauski, Korennykh, Bernales, Shokat, Walter: "Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic." in: eLife, Vol. 4, (2016) (PubMed).

Turner, Moake: "Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings." in: PloS one, Vol. 10, Issue 10, pp. e0140740, (2016) (PubMed).

Torregrosa-Muñumer, Goffart, Haikonen, Pohjoismäki: "Low doses of ultraviolet radiation and oxidative damage induce dramatic accumulation of mitochondrial DNA replication intermediates, fork regression, and replication initiation shift." in: Molecular biology of the cell, Vol. 26, Issue 23, pp. 4197-208, (2015) (PubMed).

Junk, Cipriano, Stampfer, Jackson: "Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells." in: PloS one, Vol. 8, Issue 2, pp. e53776, (2013) (PubMed).

Xu, Grande, Garofalo, Neamati: "Discovery of a novel orally active small-molecule gp130 inhibitor for the treatment of ovarian cancer." in: Molecular cancer therapeutics, Vol. 12, Issue 6, pp. 937-49, (2013) (PubMed).

Zong, Satoh, Yu, Siu, Ng, Chan, Tanner, Yu: "TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane." in: PloS one, Vol. 7, Issue 1, pp. e29995, (2012) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Synonyms: Mouse Antibody, Gt-a-Mouse IgA, Mouse Antibody in Goat, Mouse Secondary Antibody