Ziege anti-Maus IgG (Fc Region) Antikörper

Produktnummer ABIN101851

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG F(c)

Produktmerkmale: Concentration Definition: by Refractometry

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against Mouse IgG F(ab).

Immunogen:

Immunogen: Mouse IgG F(c) fragment

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 85 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen

Li, Wang, Li: "Kinesin family member 20B regulates tongue cancer progression by promoting cell proliferation." in: Molecular medicine reports, Vol. 19, Issue 3, pp. 2202-2210, (2019) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-Mouse IgG F(c) fragment Antibody, goat anti-Mouse IgG Fc fragment Antibody, rabbit anti Mouse IgG Fc

Background: Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.