Anti-Swine IgG whole molecule antibody generated in goat detects specifically Swine IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Immunogen
Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.
ELISA, IHC, WB
Polyclonal
IgG
unconjugated
F(ab')2 fragment
Applikationshinweise
This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Restore with deionized water (or equivalent)
Konzentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Gentamicin sulfate
Handhabung
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.