Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (Texas Red (TR))

Produktnummer ABIN101949

Hersteller Produkt- Nr.: 611-7902

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Esel

Klonalität: Polyklonal

Konjugat: Texas Red (TR)

Applikation: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 611-7902

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody Texas Red™ Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, and Rabbit Serum.

Produktmerkmale: Anti-Rabbit IgG (H&L) Biotin Antibody generated in donkey detects reactivity to Rabbit IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Rabbit IgG whole molecule

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Rabbit IgG (H&L) Texas Red Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Li, Chen, Li: "Butyrate alleviates metabolic impairments and protects pancreatic β cell function in pregnant mice with obesity." in: International journal of clinical and experimental pathology, Vol. 6, Issue 8, pp. 1574-84, (2014) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.