Anti-Rabbit IgG antibody generated in donkey detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Aufreinigung
Preadsorption: Solid phase absorption
Sterilität
Sterile filtered
Immunogen
Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Anti-Rabbit IgG (H&l) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung
4 °C
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Hua, Han, Zhou, Kim, Shi, Abeysirigunawardena, Jain, Singh, Aggarwal, Woodson, Ha: "An improved surface passivation method for single-molecule studies." in: Nature methods, Vol. 11, Issue 12, pp. 1233-6, (2015) (PubMed).