Ziege anti-Kaninchen IgG (Fc Region) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN102070

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: IgG F(c)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rabbit IgG F(c) fragment

Isotyp: IgG

Labeling Ratio: 3.5

Anwendungsinformationen

Applikationshinweise:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat anti-Rabbit IgG F(c) Antibody fluorescein Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody fluorescein Conjugation, Goat anti-Rabbit IgG F(c) FITC Conjugated Antibody

Background: Anti-Rabbit IgG F(c) Fluorescein antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.