ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
Spezifität
IgG F(c)
Produktmerkmale
Anti-Rabbit F(c) peroxidase conjugated antibody generated in goat detects specifically rabbit IgG F(c). This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Immunogen
Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG fragment in goat.
Antibody Anti-Rabbit IgG F(c) peroxidase conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Restore with deionized water (or equivalent)
Konzentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Gentamicin sulfate
Handhabung
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C
Hernández-Vásquez, Adame-García, Hamoud, Chidiac, Reyes-Cruz, Gratton, Côté, Vázquez-Prado: "Cell adhesion controlled by adhesion G protein-coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising intersectins and Elmo-Dock." in: The Journal of biological chemistry, Vol. 292, Issue 29, pp. 12178-12191, (2017) (PubMed).