Ziege anti-Ratte IgG (Heavy & Light Chain) Antikörper

Produktnummer ABIN102111

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG (H&L)

Produktmerkmale: Concentration Definition: by Refractometry

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rat IgG and Rat Serum.

Immunogen:

Immunogen: Rat IgG whole molecule

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Rat IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid, Lyophilized

Rekonstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-Rat IgG Antibody, goat anti Rat IgG

Background: Anti-Rat IgG (H&L) generated in goat detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.