Esel anti-Schaf IgG (Heavy & Light Chain) Antikörper (Biotin)

Produktnummer ABIN102222

Hersteller Produkt- Nr.: 613-7602

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Schaf

Wirt: Esel

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 613-7602

Hersteller: Rockland

Verwendungszweck: Sheep IgG (H&L) Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum.

Produktmerkmale: Anti-Sheep IgG (H&L) antibody generated in donkey detects specifically sheep IgG heavy and light chains (H&L). This Anti sheep antibody is conjugated to alkaline phosphatase and this secondary antibody is suitable for immunoblotting and immunoassays. Anti-Sheep IgG (H&L) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

Aufreinigung: Anti-Sheep IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Sheep IgG whole molecule was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Sheep IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Sheep IgG antibody generated in donkey detects specifically Sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.