Esel anti-Schaf IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN102234

Hersteller Produkt- Nr.: 613-703-168

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Schaf

Wirt: Esel

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 613-703-168

Hersteller: Rockland

Verwendungszweck: Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.

Produktmerkmale: Anti-Sheep IgG (H&L) fluorescein Antibody generated in donkey detects reactivity to sheep IgG.

Aufreinigung: Anti-Sheep IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Sheep IgG (H&L) peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:65,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Anti-Sheep IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically sheep IgG. This secondary peroxidase conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.