Kaninchen anti-Maus IgG1 (Heavy Chain) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN102336

Produktdetails

Target: IgG1

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: IgG1

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Isotyp: IgG

Labeling Ratio: 3.9

Anwendungsinformationen

Applikationshinweise:

Application Note: Mouse IgG1 secondary antibody conjugated to FITC is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This IgG1 antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: User Optimized

IF Microscopy Dilution: 1:500 - 1:2,500

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG1

Abstract: IgG1 Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody fluorescein Conjugation, Rabbit Anti-Mouse IgG1 FITC Conjugated Antibody

Background: Anti-Mouse IgG1 Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.