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Kaninchen anti-Maus IgG2b (Heavy Chain) Antikörper (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
Produktnummer ABIN102400
  • Target Alle IgG2b Produkte
    IgG2b
    Bindungsspezifität
    • 43
    • 16
    • 5
    • 3
    • 3
    Heavy Chain
    Reaktivität
    • 88
    • 23
    Maus
    Wirt
    • 59
    • 31
    • 12
    • 8
    • 1
    Kaninchen
    Klonalität
    • 70
    • 20
    Polyklonal
    Konjugat
    • 19
    • 12
    • 10
    • 9
    • 8
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Applikation
    • 55
    • 47
    • 35
    • 30
    • 29
    • 20
    • 9
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Spezifität
    IgG2b
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG2b heavy chain

    Isotyp
    IgG
    Labeling Ratio
    4.0
  • Applikationshinweise

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Kommentare

    Excitation/Emission wavelength: 494 nm/514 nm

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Product is photosensitive and should be protected from light.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgG2b
    Abstract
    IgG2b Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2b Antibody FITC Conjugation

    Background: Anti-Mouse IgG2b Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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