Kaninchen anti-Maus IgG2b (Heavy Chain) Antikörper (FITC) - Preadsorbed
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- Target Alle IgG2b Produkte
- IgG2b
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Bindungsspezifität
- Heavy Chain
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Reaktivität
- Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- IgG2b
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG2b heavy chain
- Isotyp
- IgG
- Labeling Ratio
- 4.0
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- Applikationshinweise
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Kommentare
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Excitation/Emission wavelength: 494 nm/514 nm
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Product is photosensitive and should be protected from light.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgG2b
- Abstract
- IgG2b Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Rabbit Anti-Mouse IgG2b (gamma 2b chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2b Antibody FITC Conjugation
Background: Anti-Mouse IgG2b Fluorescein Antibody generated in rabbit detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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