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Ziege anti-Human IgM Antikörper (HRP)

Rockland 609-1307 ELISA, IHC, WB Polyclonal IgG HRP
Rockland
Produktnummer ABIN102628
Hersteller Produkt- Nr.: 609-1307
  • Target Alle IgM Produkte
    IgM
    Reaktivität
    • 225
    • 162
    • 91
    • 42
    • 34
    • 34
    • 29
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Human
    Wirt
    • 372
    • 231
    • 87
    • 22
    • 14
    • 5
    • 4
    • 1
    • 1
    Ziege
    Klonalität
    • 539
    • 96
    Polyklonal
    Konjugat
    • 122
    • 86
    • 85
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    HRP
    Applikation
    • 384
    • 289
    • 282
    • 242
    • 182
    • 137
    • 91
    • 91
    • 64
    • 62
    • 54
    • 53
    • 45
    • 30
    • 18
    • 17
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Hersteller Produkt- Nr.
    609-1307
    Hersteller
    Rockland
    Verwendungszweck
    Human IgM (mu chain) Antibody Peroxidase Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum.  No reaction was observed against other human heavy or light chain proteins.
    Produktmerkmale
    Anti-Human IgA Peroxidase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    Aufreinigung
    Human IgM (mu chain) Antibody, Peroxidase Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen
    Optional[Immunogen]: Human IgM whole molecule
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Human IgM (mu heavy chain) Peroxidase has been tested by ELISA and is suitable for western blot, immunohistochemistry, and immunoassays where specificity to the immunoglobulin mu chain region is desired. Optimal concentrations in immunoassays should be determined by the researcher. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:180,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Konservierungsmittel
    Gentamicin sulfate
    Vorsichtsmaßnahmen
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Crawford, Tempel, Streblow, Kreklywich, Smith, Picker, Nelson, Caposio: "Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice." in: Scientific reports, Vol. 7, Issue 1, pp. 937, (2018) (PubMed).

    Yang, Jacobs, McCallen, Moore, Huckaby, Edelstein, Lai: "Analysis of Pre-existing IgG and IgM Antibodies against Polyethylene Glycol (PEG) in the General Population." in: Analytical chemistry, Vol. 88, Issue 23, pp. 11804-11812, (2018) (PubMed).

    Zupancic, Blejec, Herman, Veber, Verbovsek, Korsic, Knezevic, Rozman, Turnsek, Gruden, Motaln: "Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approach." in: Radiology and oncology, Vol. 48, Issue 3, pp. 257-66, (2014) (PubMed).

  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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