Kaninchen anti-Human IgM (Heavy Chain) Antikörper (FITC)
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- Target Alle IgM Produkte
- IgM
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Bindungsspezifität
- Heavy Chain
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- IgM µ chain
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG.
- Immunogen
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Immunogen: Human IgM mu heavy chain
- Isotyp
- IgG
- Labeling Ratio
- 4.3
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- Applikationshinweise
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Application Note: Anti-Human IgM (mu heavy chain) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Kommentare
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Excitation/Emission wavelength: 494 nm/514 nm
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Product is photosensitive and should be protected from light.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgM
- Abstract
- IgM Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: rabbit anti-Human IgM (mu chain) Fluorescein Conjugated Antibody, rabbit anti-Human IgM mu chain FITC Conjugated Antibody, rabbit anti-Human IgM mu chain Antibody Fluorescein Conjugation, rabbit anti-Human IgM (mu chain) Antibody FITC Conjugation
Background: Anti-Human IgM (mu heavy chain) Fluorescein generated in rabbit detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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