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Kaninchen anti-Human IgM (Heavy Chain) Antikörper (FITC)

FACS, FLISA, FM Polyclonal IgG FITC
Produktnummer ABIN102634
  • Target Alle IgM Produkte
    IgM
    Bindungsspezifität
    • 162
    • 122
    • 87
    • 57
    • 26
    • 9
    • 7
    • 3
    • 1
    • 1
    Heavy Chain
    Reaktivität
    • 226
    • 159
    • 89
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    • 1
    Human
    Wirt
    • 365
    • 230
    • 89
    • 21
    • 14
    • 5
    • 4
    • 1
    • 1
    Kaninchen
    Klonalität
    • 533
    • 95
    Polyklonal
    Konjugat
    • 122
    • 85
    • 84
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    FITC
    Applikation
    • 377
    • 282
    • 261
    • 221
    • 130
    • 124
    • 91
    • 91
    • 63
    • 61
    • 53
    • 53
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Spezifität
    IgM µ chain
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgM and Human Serum.  No reaction was observed against Human IgG.
    Immunogen

    Immunogen: Human IgM mu heavy chain

    Isotyp
    IgG
    Labeling Ratio
    4.3
  • Applikationshinweise

    Application Note: Anti-Human IgM (mu heavy chain) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Kommentare

    Excitation/Emission wavelength: 494 nm/514 nm

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Product is photosensitive and should be protected from light.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: rabbit anti-Human IgM (mu chain) Fluorescein Conjugated Antibody, rabbit anti-Human IgM mu chain FITC Conjugated Antibody, rabbit anti-Human IgM mu chain Antibody Fluorescein Conjugation, rabbit anti-Human IgM (mu chain) Antibody FITC Conjugation

    Background: Anti-Human IgM (mu heavy chain) Fluorescein generated in rabbit detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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