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Kaninchen anti-Human IgM Antikörper (Alkaline Phosphatase (AP))

Rockland 609-4531 ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rockland
Produktnummer ABIN102641
Hersteller Produkt- Nr.: 609-4531
  • Target Alle IgM Produkte
    IgM
    Reaktivität
    • 225
    • 162
    • 91
    • 42
    • 34
    • 34
    • 29
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Human
    Wirt
    • 373
    • 230
    • 87
    • 22
    • 14
    • 5
    • 4
    • 1
    • 1
    Kaninchen
    Klonalität
    • 539
    • 96
    Polyklonal
    Konjugat
    • 122
    • 86
    • 86
    • 66
    • 33
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Alkaline Phosphatase (AP)
    Applikation
    • 384
    • 289
    • 282
    • 242
    • 182
    • 137
    • 91
    • 91
    • 64
    • 62
    • 53
    • 53
    • 45
    • 30
    • 18
    • 17
    • 10
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
    Hersteller Produkt- Nr.
    609-4531
    Hersteller
    Rockland
    Verwendungszweck
    Human IgM (Fc5u) Antibody Alkaline Phosphatase Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgM and Human Serum.   Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
    Produktmerkmale
    Anti-Human IgG F(ab')2 Alkaline Phosphatase Antibody generated in rabbit recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    Aufreinigung
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterilität
    Sterile filtered
    Immunogen
    Optional[Immunogen]: Human IgM Fc5mu fragment
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: Anti-Human IgM Fc5μ Alkaline Phosphatase conjugate has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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