Ziege anti-Affe IgM (Chain mu) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN102651

Produktdetails

Target: IgM

Bindungsspezifität: Chain mu

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgM µ chain

Produktmerkmale: Anti-Monkey IgM (mu chain) Antibody generated in goat detects specifically mouse IgM whole molecule. Anti-Monkey IgM (mu chain) Antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Monkey IgM (mu chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Monkey IgM whole molecule

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Monkey IgM (mu chain) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:3,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat Anti-Monkey IgM Antibody alkaline phosphatase Conjugation, Goat Anti-Monkey IgM alk phos Conjugated Antibody

Background: Anti-Monkey IgM (mu chain) alkaline phosphatase antibody generated in goat specifically detects monkey IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.