Ziege anti-Maus IgM (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN102676

Produktdetails

Target: IgM

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgM µ chain

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgM whole molecule

Isotyp: IgG

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgM (mu chain) biotin conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Abstract: IgM Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat Anti-Mouse IgM (mu chain) Antibody Biotin Conjugated, Goat Anti Mouse IgM mu chain Biotin Conjugated Antibody

Background: Anti-Mouse IgM (mu chain) biotin conjugated antibody generated in goat detects specifically mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.