Ziege anti-Kaninchen IgM (Heavy Chain) Antikörper (FITC)
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- Target Alle IgM Produkte
- IgM
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Bindungsspezifität
- Heavy Chain
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Reaktivität
- Kaninchen
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- IgM µ chain
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Rabbit IgM and Rabbit Serum. No reaction was observed against Rabbit IgG.
- Immunogen
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Immunogen: Rabbit IgM mu heavy chain
- Isotyp
- IgG
- Labeling Ratio
- 4.2
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- Applikationshinweise
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Kommentare
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Excitation/Emission wavelength: 494 nm/514 nm
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Product is photosensitive and should be protected from light.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgM
- Abstract
- IgM Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: goat anti-Rabbit IgM (mu chain) Antibody fluorescein Conjugated, goat anti-Rabbit IgM mu chain FITC Conjugated Antibody
Background: Anti-Rabbit IgM mu chain antibody raised in goat specifically detects rabbit IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgM is conjugated to Fluorescein.
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