Esel anti-Huhn IgG (Heavy & Light Chain) Antikörper (FITC) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Huhn
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Wirt
- Esel
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.
- Kreuzreaktivität
- Huhn
- Produktmerkmale
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Aufreinigung
- Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads
- Immunogen
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Immunogen: Chicken IgG whole molecule
- Isotyp
- IgG
- Labeling Ratio
- 3.0
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- Applikationshinweise
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Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Kommentare
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Excitation/Emission wavelength: 494 nm/514 nm
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Dilute only prior to immediate use. - Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Donkey anti-Chicken IgG Antibody Fluorescein Conjugation, Donkey anti-Chicken IgG FITC Conjugated Antibody
Background: Anti-Chicken IgG Fluorescein Antibody generated in donkey detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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