Esel anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper - Preadsorbed

Produktnummer ABIN1043951

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Meerschweinchen

Wirt: Esel

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum.

Kreuzreaktivität: Meerschweinchen

Aufreinigung: Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads

Sterilität: Sterile filtered

Immunogen:

Immunogen: Guinea Pig IgG whole molecule

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Guinea Pig IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:25,000 - 1:75,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Konservierungsmittel: Without preservative

Handhabung: Centrifuge product if not completely clear after standing at room temperature.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Donkey Anti-Guinea Pig IgG (H&L) Antibody Pre-Adsorbed, Donkey Anti Guinea Pig IgG

Background: Anti-Guinea Pig IgG Antibody generated in donkey detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.