Ziege anti-Maus IgG1 (Heavy Chain) Antikörper (Biotin) - Preadsorbed
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- Target Alle IgG1 Produkte
- IgG1
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Bindungsspezifität
- Heavy Chain
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Reaktivität
- Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Biotin
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Applikation
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Spezifität
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse Serum and Mouse IgG.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - Kreuzreaktivität
- Maus
- Produktmerkmale
- Biotin Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Aufreinigung
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG1 Biotin Conjugated Secondary Antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Aliquot to Avoid repeated freezing and thawing.
- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
- Haltbarkeit
- 12 months
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- Target
- IgG1
- Abstract
- IgG1 Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Goat anti-mouse IgG1 antibody biotin conjugation, goat anti-mouse IgG1 (gamma 1) biotin conjugated antibody
Background: Anti-Mouse IgG1 Biotin Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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