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Ziege anti-Maus IgG2a (Heavy Chain) Antikörper (FITC) - Preadsorbed

FACS, IHC, WB, FLISA, FM Polyclonal IgG FITC
Produktnummer ABIN1118478
  • Target Alle IgG2a Produkte
    IgG2a
    Bindungsspezifität
    • 50
    • 15
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    Heavy Chain
    Reaktivität
    • 101
    • 18
    • 1
    Maus
    Wirt
    • 56
    • 29
    • 17
    • 13
    • 5
    Ziege
    Klonalität
    • 71
    • 30
    Polyklonal
    Konjugat
    • 22
    • 13
    • 12
    • 10
    • 9
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Applikation
    • 58
    • 49
    • 37
    • 32
    • 23
    • 18
    • 11
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Spezifität
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.
    Kreuzreaktivität
    Maus
    Produktmerkmale
    Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG2a heavy chain

    Immunogen Type: Native Protein

    Isotyp
    IgG
    Labeling Ratio
    5.2
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:200 - 1:500

    Western Blot Dilution: 1:2,000 - 1:10,000

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Kommentare

    Excitation/Emission wavelength: 494 nm/514 nm

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.7 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Product is photosensitive and should be protected from light.
    Centrifuge product if not completely clear after standing at room temperature.
    Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Haltbarkeit
    12 months
  • Musante, Li, Kanyo, Lam, Colangelo, Cheng, Brody, Greengard, Le Novère, Nairn: "Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition." in: eLife, Vol. 6, (2018) (PubMed).

    Carlyle, Kitchen, Kanyo, Voss, Pletikos, Sousa, Lam, Gerstein, Sestan, Nairn: "A multiregional proteomic survey of the postnatal human brain." in: Nature neuroscience, Vol. 20, Issue 12, pp. 1787-1795, (2017) (PubMed).

    Andrade, Musante, Horiuchi, Matsuzaki, Brody, Wu, Greengard, Taylor, Nairn: "ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase)." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 10, pp. 2709-2722, (2017) (PubMed).

  • Target
    IgG2a
    Abstract
    IgG2a Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: goat anti-MOUSE IgG2a Antibody fluorescein Conjugation, goat anti-Mouse IgG2a (gamma 2a) FITC Conjugated Antibody

    Background: Anti-Mouse IgG2a Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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