Ziege anti-Maus IgG1 (Heavy Chain) Antikörper (Atto 647N) - Preadsorbed

Produktnummer ABIN1118519

Produktdetails

Target: IgG1

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Atto 647N

Applikation: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Kreuzreaktivität: Maus

Produktmerkmale: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 1.3

Anwendungsinformationen

Applikationshinweise:

Application Note: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Flow Cytometry Dilution: 1:500 - 1:2,500

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare:

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Referenzen

Schilling, Ali, Leonhardt, Borst, Pujol-Martí: "Transcriptional control of morphological properties of direction-selective T4/T5 neurons in Drosophila." in: Development (Cambridge, England), Vol. 146, Issue 2, (2019) (PubMed).

Hruska, Henderson, Le Marchand, Jafri, Dalva: "Synaptic nanomodules underlie the organization and plasticity of spine synapses." in: Nature neuroscience, Vol. 21, Issue 5, pp. 671-682, (2019) (PubMed).

Antigendetails

Target: IgG1

Abstract: IgG1 Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat anti-mouse IgG1 antibody ATTO647N conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 647N conjugated antibody

Background: Anti-Mouse IgG1 ATTO 647N Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.