Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Ferret IgM in astandard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,500 of the reconstitutionconcentration is suggested for this product.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Do not add Sodium azide. Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.