It has been selected for its ability to recognize IgG in immunohistochemical staining and Western blotting.
Aufreinigung
Affinity Chromatography
Immunogen
Native Protein IgG Immunoglobulin G (IgG) is one of the most abundant proteins in serum and the major components of the immune system. IgG is important for our defence against microorganisms and the molecules, which are produced by B lymphocytes as a part of our adaptive immune response. By binding many kinds of pathogen—representing viruses, bacteria, and fungi—IgG protects the body from infection. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. The antibody is a rabbit polyclonal antibody raised against IgG.
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Konzentration
200 µg/mL
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freeze/thaw cycles
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.