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Ziege anti-Human Ig (Chain kappa), (Light Chain) Antikörper (Biotin)

ELISA, IHC, WB, IEP, IM Polyclonal Biotin F(ab')2 fragment

Produktnummer ABIN117713

Datenblatt (PDF)

Target Alle Ig Produkte

Ig

Fragment

F(ab')2 fragment
Bindungsspezifität
96

88

68

32

4

4

2

1

1

1
Chain kappa, Light Chain
Reaktivität
115

67

28

19

9

4

2

1

1

1

1

1

1

1

1

1
Human
Wirt
105

80

36

23

2

1

1

1
Ziege
Klonalität
117

106
Polyklonal
Konjugat
60

32

25

21

17

16

10

10

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Biotin
Applikation
169

134

87

71

42

26

25

15

12

10

10

6

3

2

2

1

1

1

1

1

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoelectrophoresis (IEP), Immunomicroscopy (IM)

Produktmerkmale

Molar Ratio: 10-20 BAC molecules per Goat IgG Fab molecule.

Aufreinigung

Immunoaffinity chromatography.

Immunogen

Human kappa (kappa) light chain.
Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin

Goat anti-Human Ig antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Human Ig antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human Ig (Heavy & Light Chain) antibody (Biotin)
ELISA, FACS, WB Polyclonal IgG Biotin

Goat anti-Human Ig antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human Ig (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal IgG HRP

Goat anti-Human Ig antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Human Ig (Chain lambda) antibody
ELISA, FACS, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human Ig (Chain lambda) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human Ig (Chain kappa) antibody (Texas Red (TR))
ELISA, FACS, IHC Polyclonal IgG Texas Red (TR)

Applikationshinweise

Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy aswell as other antibody based assays using streptavidin or avidin conjugates requiringextremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency,high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 8,500 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Rekonstitution

Restore with 1.0 mL of deionized water (or equivalent).

Konzentration

1.0 mg/mL (by UV absorbance at 280 nm)

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target

Ig

Andere Bezeichnung

Immunoglobulin (Ig Produkte)