Maus anti-Human Ig (Chain lambda), (Light Chain) Antikörper (FITC)

Produktnummer ABIN118381

Kurzübersicht für Maus anti-Human Ig (Chain lambda), (Light Chain) Antikörper (FITC) (ABIN118381)

Target: Ig

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS)

Klon: Mc24-IC6

Isotyp: IgG1

Produktdetails

Bindungsspezifität: Chain lambda, Light Chain

Reaktivität: Human

Wirt: Maus

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Anwendungsinformationen

Applikationshinweise: Flow cytometry: use neat antibody to label 10^6 cells or 10 μL washed whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Ig

Andere Bezeichnung: Immunoglobulin

Hintergrund: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5 kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L, Lambda, 0.4-4.2 mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).