Ziege anti-Maus IgG, IgM Antikörper (TRITC) - Preadsorbed

Produktnummer ABIN129738

Hersteller Produkt- Nr.: 610-100-115

Produktdetails

Target: IgG, IgM

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: TRITC

Applikation: Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Hersteller Produkt- Nr.: 610-100-115

Hersteller: Rockland

Verwendungszweck: Mouse IgG + IgM (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM.  No reaction was observed against bovine, horse or human serum proteins.

Produktmerkmale: Anti-Mouse IgG3 Rhodamine Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).

Aufreinigung: Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse IgG and IgM whole molecules

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-MOUSE IgG + IgM (H&L) (GOAT) Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgG and IgM antibody is suitable for immunoassays including western blot and ELISA. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG, IgM

Andere Bezeichnung: IgG,IgM (IgG, IgM Produkte)

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Rhodamine Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.