Ziege anti-Maus IgG, IgM Antikörper (HRP) - Preadsorbed

Produktnummer ABIN129742

Hersteller Produkt- Nr.: 610-103-115

Produktdetails

Target: IgG, IgM

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 610-103-115

Hersteller: Rockland

Verwendungszweck: Mouse IgG + IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against No reaction was observed against bovine, horse or human serum proteins.

Produktmerkmale: Anti-Mouse IgG3 Peroxidase Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).

Aufreinigung: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Mouse IgG and IgM whole molecule was produced by repeated immunization with Mouse IgG and IgM whole molecule in goat.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG and IgM Peroxidase conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:40,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG, IgM

Andere Bezeichnung: IgG,IgM (IgG, IgM Produkte)

Substanzklasse: Antibody

Hintergrund: Anti-Mouse IgG and IgM whole molecule peroxidase conjugated antibody generated in goat detects specifically Mouse IgG and IgM whole molecule. This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.