Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (PE) - Preadsorbed

Produktnummer ABIN129765

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Spezifität: IgG (H&L)

Kreuzreaktivität: Kaninchen

Produktmerkmale: Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Concentration Definition: by absorbance = 82.0 at 565 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Rabbit IgG (H&L) Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.

Lagerung: RT,4 °C

Haltbarkeit: 12 months

Referenzen

Yasunaga, Ohtsubo, Ohno, Saeki, Kurogi, Tanaka-Okamoto, Ishizaki, Shirai, Mihara, Brock, Miyoshi, Takihara: "Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4." in: Molecular and cellular biology, Vol. 33, Issue 4, pp. 644-60, (2013) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit Phycoerythrin conjugated, Rabbit IgG (H&L) Antibody in goat, Goat IgG (H&L) Phycoerythrin conjugated Secondary Antibody.

Background: Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.