FACS, ELISA
Polyclonal
IgG
Biotin
F(ab')2 fragment
Applikationshinweise
Applications: ELISA - Quality tested FLISA - Quality tested FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Costim - Reported in literature
Working Dilutions: ELISA Purified (UNLB) antibody 1 g/mL AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 20,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Konservierungsmittel
Without preservative
Handhabung
Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.