ELISA, WB, ICC, IHC (p), DB
Monoclonal
NI 194-3 (A89-040)
IgG1
HRP
Applikationshinweise
Applications: ELISA - Quality tested , FLISA - Quality tested ELISPOT - Reported in literature , FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature , Sep - Reported in literature , Depletion - Reported in literature
Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:16,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Konservierungsmittel
Without preservative
Handhabung
Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C
Pöhler, Emrich, Jordan, Schäfer, Stubenrauch, Staack, Zach, Meir, Faigle: "Comparison of assay formats used for the detection of pre-existing anti-drug antibodies against monoclonal antibodies." in: Bioanalysis, Vol. 14, Issue 13, pp. 923-933, (2022) (PubMed).