After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent and conjugated to fluorescein isothiocyanate (FITC).
Aufreinigung
egg yolk-derived purified IgY, affinity purified
Immunogen
Chickens were immunized with highly purified rabbit IgG isolated from normal pooled rabbit serum.
Optimal working dilutions should be determined for your particular assay conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.0 mg/mL
Buffer
Phosphate buffered saline, 50 % glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Product is photosensitive and should be protected from light. Do not freeze-thaw often