Ziege anti-Kaninchen Ig Antikörper (CypHer5E)

Produktnummer ABIN1742571

Produktdetails

Target: Ig

Fragment: Fab fragment

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: CypHer5E

Applikation: Immunocytochemistry (ICC)

Aufreinigung: affinity purified Fab fragments. Rabbit serum albumin was added for stabilization.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. Application Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µL for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay. ICC: image

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives.

Antigendetails

Target: Ig

Abstract: Ig Produkte