Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibodybased assays using streptavidin or avidin conjugates: ELISA: 1/60,000-1/600,000 Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL of deionized water
Konzentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containg 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 °C prior to restoration. For extended storage after reconstitution, mix product with glycerol to 50 % final, then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.