Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (PE)

Produktnummer ABIN2145659

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ziege

Konjugat: PE

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS)

Produktmerkmale: High purity Rabbit IgG(H+L) is prepared from serum of healthy rabbit. Affinity purified Goat anti- Rabbit IgG(H+L) isolated from pooled antiserum from goats hyperimmunized with purified Rabbit IgG(H+L).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
• Add 2 mL1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add appropriate amount of fluorescent-labeled Goat anti Mouse IgG and incubate for 20 minutes away from light at room temperature.
• Repeat step 5.
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: aqueous buffer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen

Sattler, El-Battrawy, Cyganek, Lang, Lan, Li, Zhao, Utikal, Wieland, Borggrefe, Zhou, Akin: "TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes." in: Scientific reports, Vol. 11, Issue 1, pp. 14689, (2021) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody