Esel anti-Maus IgG (Heavy & Light Chain) Antikörper (PE) - Preadsorbed

Produktnummer ABIN2669889

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Esel

Klonalität: Polyklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Mouse IgG and Mouse Serum.

Kreuzreaktivität: Maus

Produktmerkmale: F(ab')2 Anti-Mouse IgG whole molecule generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: F(ab')2 Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 5.6

Anwendungsinformationen

Applikationshinweise:

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Kommentare:

Post Translational Modification: Phosphorylation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.

Lagerung: RT,4 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Donkey F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Mouse IgG PE Conjugated Antibody

Background: F(ab')2 Anti-Mouse IgG whole molecule generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.