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Maus anti-Human IgG (Fc Region) Antikörper (FITC)

Name: Maus anti-Human IgG (Fc Region) Antikörper (FITC)
Brand: antibodies-online
SKU: ABIN302015
Price: 402.57 EUR
Availability: InStock

FACS Monoclonal EM-07 IgG1 FITC

IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B). (Maus anti-Human IgG (Fc Region) Antikörper (FITC))

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) FITC antibody (3 μL reagent / 100 μL of peripheral whole blood). (Maus anti-Human IgG (Fc Region) Antikörper (FITC))

FACS

Flow cytometry analysis (surface staining) of human peripheral blood using anti-IgG (Fc), clone EM-07, FITC. (Maus anti-Human IgG (Fc Region) Antikörper (FITC))

FACS

Separation of human IgG (Fc) positive CD19 negative lymphocytes (red-filled) from CD19 positive IgG (Fc) negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) FITC antibody (3 μL reagent / 100 μL of peripheral whole blood). (Maus anti-Human IgG (Fc Region) Antikörper (FITC))

FACS

4 Abbildungen

Produktnummer ABIN302015

Datenblatt (PDF)

Kurzübersicht für Maus anti-Human IgG (Fc Region) Antikörper (FITC) (ABIN302015)

Target

IgG

Klonalität

Monoklonal

Konjugat

FITC

Applikation

2120
2053
1899
1874
999
920
896
520
424
234
234
149
102
98
90
84
51
33
30
28
25
20
15
11
9
8
7
7
5
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1

Flow Cytometry (FACS)

Klon

EM-07

Isotyp

IgG1

Bindungsspezifität
2397

516

417

146

130

128

66

29

28

28

21

9

5

5

2

2

2

2

2

1

1

1
Fc Region
Reaktivität
751

706

609

474

410

244

213

176

146

141

124

110

107

101

83

62

48

33

28

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Human
Wirt
1787

1583

569

283

122

112

41

32

22

21

15

11

11
Maus

Verwendungszweck

Anti-Hu IgG (Fc) FITC

Spezifität

The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

Kreuzreaktivität (Details)

Human, Other not determined

Aufreinigung

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

Fusion protein of human IgG Fc fragment.
Mouse anti-Human IgG (Fc Region) antibody
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated

Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated

Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG1 kappa unconjugated

Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG1 kappa PE

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Mouse anti-Human IgG (Fc Region) antibody (HRP)
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP

Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG Texas Red (TR)

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Applikationshinweise

Flow cytometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.1 mg/mL

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.
Avoid prolonged exposure to light.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

IgG

Substanzklasse

Antibody

Hintergrund

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

Molekulargewicht

150 kDa