Esel anti-Kaninchen IgG (Fc Region) Antikörper - Preadsorbed

Produktnummer ABIN349618

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Kaninchen

Wirt: Esel

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG F(c)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose

Immunogen:

Immunogen: Rabbit IgG F(c) fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Rabbit IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:100,000

Western Blot Dilution: 1:2,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Donkey Anti Rabbit IgG F(c) Antibody, Donkey Anti-Rabbit IgG Fc Fragment Antibody, Donkey Anti Rabbit IgG Fc Antibody

Background: Anti-Rabbit IgG F(c) generated in donkey is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.