ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
Spezifität
Reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry.
Produktmerkmale
Source: Pooled antisera from goats hyperimmunized with mouse IgM paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Aufreinigung
Affinity chromatography on pooled human Igs with light chains covalently linked to agarose
Working Dilution: <= 1 µg/10^6 cells or 1:5,000-1:20,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid cycles of freezing and thawing.
Lagerung
4 °C
Andersson, Dahlgren: "2-Hydroxyethyl methacrylate (HEMA) promotes IgG but not IgM antibody production in vivo in mice." in: European journal of oral sciences, Vol. 119, Issue 4, pp. 305-9, (2011) (PubMed).
Palmer, Stasko, Waters, Thacker: "Examination of the reticular epithelium of the bovine pharyngeal tonsil." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 294, Issue 11, pp. 1939-50, (2011) (PubMed).